Mouse TNF Alpha/TNF ELISA Kit

96T/1880

Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor

Expression system for standard: E.coli; Immunogen sequence: L80-L235

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

15.6pg/ml-1000pg/ml

TNF

Tumor necrosis factor

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Catalog number	Description	Quantity
EK0527-CAP	96-well plate precoated with anti- mouse TNF alpha antibody	1
EK0527-ST	Lyophilized recombinant mouse TNF alpha standard	10ng/tubex2
EK0527-DA	Biotinylated anti- mouse TNF alpha antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	18	50	299	16	53	305
Standard deviation	0.82	3.9	22.12	0.91	4.39	24.4
CV(%)	4.6%	7.8%	7.4%	5.7%	8.3%	8%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.007	0.072	0.139	0.260	0.552	0.920	1.748	2.358

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h