Mouse IL-1 alpha/IL1A ELISA Kit

96T/2280

Hematopoietin 1; IL 1 alpha; IL 1A; IL1; IL-1; IL1 ALPHA; IL-1 alpha; IL1A; IL1F1; IL-1α; Interleukin 1 alpha; interleukin 1, alpha; IL-1A

Expression system for standard: E.coli; Immunogen sequence: S115-S270

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

4.7pg/ml-300pg/ml

IL1A

Interleukin-1 alpha

P01582

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.

Catalog number	Description	Quantity
EK0391-CAP	96-well plate precoated with anti- mouse IL-1 alpha antibody	1
EK0391-ST	Lyophilized recombinant mouse IL-1 alpha standard	10ng/tubex2
EK0391-DA	Biotinylated anti- mouse IL-1 alpha antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	15	63	151	16	60	155
Standard deviation	1	2.7	6.64	1.18	3.3	7.28
CV(%)	6.7%	4.3%	4.4%	7.4%	5.5%	4.7%

Concentration(pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.022	0.085	0.155	0.282	0.519	1.038	1.684	2.466

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h