Mouse IL6 ELISA Kit

96T/2280

B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6

Expression system for standard: E.coli; Immunogen sequence: F25-T211

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

15.6pg/ml-1000pg/ml

IL6

interleukin 6

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

Catalog number	Description	Quantity
EK0411-CAP	Anti-Mouse IL6 Pre-coated 96-well strip microplate	1
EK0411-ST	Mouse IL6 Standard	10ng/tube×2
EK0411-DA	Mouse IL6 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	36	159	436	36	147	482
Standard deviation	2.41	7.79	34.94	2.91	7.93	40.48
CV(%)	6.7%	4.9%	7.2%	8.1%	5.4%	8.4%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.032	0.085	0.141	0.237	0.452	0.716	1.270	1.981

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h