Mouse IL-1 beta/IL1B ELISA Kit

96T/2280

IL1 beta; Catabolin; IL 1; IL 1 beta; IL1 BETA; IL-1 beta; IL1- beta; IL1B; IL1beta; IL1F2; IL-1β; Interleukin 1 beta; interleukin 1; beta; Pro IL-1β; IL 1B; IL-1B

Expression system for standard: E.coli; Immunogen sequence: V118-S269

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

12.5pg/ml-800pg/ml

IL1B

Interleukin-1 beta

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Catalog number	Description	Quantity
EK0394-CAP	96-well plate precoated with anti- mouse IL-1 beta antibody	1
EK0394-ST	Lyophilized recombinant mouse IL-1 beta standard	10ng/tubex2
EK0394-DA	Biotinylated anti- mouse IL-1 beta antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	33	211	483	36	217	481
Standard deviation	1.74	10.33	34.29	2.19	14.75	35.59
CV(%)	5.3%	4.9%	7.1%	6.1%	6.8%	7.4%

Concentration(pg/ml)	0	12.5	25	50	100	200	400	800
O.D.	0.063	0.107	0.143	0.214	0.390	0.702	1.285	2.092

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h