Mouse BDNF ELISA Kit

96T/1880

Abrineurin; BDNF

Expression system for standard: NS0; Immunogen sequence: H129-R247

There is no detectable cross-reactivity with other relevant proteins.

<15pg/ml

31.2pg/ml-2000pg/ml

BDNF

Brain-derived neurotrophic factor

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

Catalog number	Description	Quantity
EK0309-CAP	Anti-Mouse Bdnf Pre-coated 96-well strip microplate	1
EK0309-ST	Mouse Bdnf Standard	10ng/tube×2
EK0309-DA	Mouse Bdnf Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	58	196	923	61	198	920
Standard deviation	4.06	14.5	46.15	5.42	18.21	60.72
CV(%)	7%	7.4%	5%	8.9%	9.2%	6.6%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.018	0.046	0.059	0.103	0.219	0.451	0.979	1.863

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA, citrate)

3.5h